Home CUSTOMERS Acta Virologica 2005 Acta Virologica Vol.49, p.227-234, 2005

Journal info


Quarterly,
Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

Published in English

Impact Factor = 1.82

Aims and Scope
Abstracted and Indexed

Select Journal







Webshop Cart

Your Cart is currently empty.

Info: Your browser does not accept cookies. To put products into your cart and purchase them you need to enable cookies.

Acta Virologica Vol.49, p.227-234, 2005

Title: INHIBITION OF MEASLES VIRUS MULTIPLICATION IN CELL CULTURE BY RNA INTERFERENCE
Author: L. HU, Z. WANG, C. HU, X. LIU, L. YAO, W. LI, Y. QI

Abstract: Measles takes a toll of one million people a year worldwide, especially in developing countries. As the so far applied vaccines were not able to control this disease, novel approaches to this problem are needed. In this study, we investigated the possibility of inhibition of Measles virus (MV) infection through RNA interference (RNAi), namely the interference with the second but main receptor of MV, signaling lymphocyte activation molecule (SLAM), in an Epstein-Barr virus (EBV)-transformed marmoset B cell line B95-8. Three potential target sequences in the SLAM gene were chosen and cloned into the pSilencer 3.0-H1 vector. The vector constructs were used for transfection of B95-8 cells in which SLAM was then detected by flow cytometry, Western blot analysis and RT-PCR both at transcription and expression levels. One of the vector constructs was found to block effectively the SLAM expression as well as MV infection. These results suggest that RNAi could silence SLAM expression and inhibit the MV infection of host cells.

Keywords: siRNAs; Measles virus; SLAM; RNAi; B95-8 cell line
Year: 2005, Volume: 49, Issue: Page From: 227, Page To: 234



download file



© AEPress s.r.o
Copyright notice: For any permission to reproduce, archive or otherwise use the documents in the ELiS, please contact AEP.