Home Neoplasma 2007 Neoplasma Vol.54, p.495-502, 2007

Journal info

6 times a year.
Founded: 1954
ISSN 0028-2685
ISSN 1338-4317 (online)

Published in English

Editorial Info
Abstracted and Indexed
Submission Guidelines

Select Journal

Webshop Cart

Your Cart is currently empty.

Info: Your browser does not accept cookies. To put products into your cart and purchase them you need to enable cookies.

Neoplasma Vol.54, p.495-502, 2007

Title: Sensitization of colorectal cancer cells to irradiation by IL-4 and IL-10 is associated with inhibition of NF-κB

Abstract: The efficiency of radiotherapy for rectal cancer treatment is limited because of radioresistance. Transcription factor nuclear factor-kappaB (NF-κB), which has antiapoptotic properties, may play an important role in this process. Recent studies indicate that behavior of the tumor is done not only by oncogenic events in tumor cells but also by microenvironment surrounding the tumor. Therefore we tested anti-inflammatory cytokines IL-4 and IL-10 occurring in tumor stroma whether they can modulate response of colorectal cancer cells to irradiation and whether this potential effect is associated with NF- κB. SW620 colorectal cancer cells were used for all experiments. Cell growth and clonogenicity were determined by cell proliferation assay and clonogenic assay, respectively. Activation of NF-κB was assessed by ELISA-based transcription factor assay and luciferase reporter assay. Apoptosis was determined by measuring caspase 3 activity. Irradiation (2–8 Gy) inhibited growth and clonogenicity of SW620 cells, induced apoptosis, and activated NF-κB, predominantly its subunits p50, p65, and RelB. IL-4 or IL-10 (1, 10, 100 ng/ml) neither inhibited growth and clonogenicity nor activated NF-κB, but they sensitized cells to irradiation in a dose dependent manner. Radiosensitization by IL-4 or IL-10 was associated with inhibition of NF-κB, predominantly its subunits p50 and p65 and increased apoptosis. In conclusion, modulation of the intestinal microenvironment, high local concentration of anti-inflammatory cytokines such as IL-4 and IL-10 may help to overcome resistance of colorectal tumors to radiotherapy. In this process NF-κB may be employed.

Keywords: IL-4, IL-10, NF-κB, p65, radiosensitivity, colorectal cancer
Year: 2007, Volume: 54, Issue: Page From: 495, Page To: 502

download file

© AEPress s.r.o
Copyright notice: For any permission to reproduce, archive or otherwise use the documents in the ELiS, please contact AEP.