Neoplasma Vol.54, p.527-535, 2007
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| Title: B-cell chronic lymphocytic leukemia-derived dendritic cells
stimulate allogeneic T-cell response and express chemokines
involved in T-cell migration |
| Author: W. LUCZYNSKI, A. STASIAK-BARMUTA, J. PISZCZ, E. ILENDO, O. KOWALCZUK, M. KRAWCZUK-RYBAK |
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Abstract: Despite discovery of new therapeutic agents, including nucleoside analogs and monoclonal antibodies, the B-cell chronic
lymphocytic leukemia (B-CLL) remains incurable. In recent years, some effort has been made in developing T-cell specific
immunity against neoplasmatic cells. Reconstitution of effective costimulation and immunological response of host T-cells
against CLL cells could be a potential approach in immunotherapeutic trials. CD40/CD40L system is involved in the survival
and proliferation of normal and neoplasmatic B-cells. Some preclinical studies have shown that CD40 stimulation can
differentiate leukemic cells into dendritic cells (DCs) and result in host response. In this study, we sought to determine
whether B-CLL cells could be turned into efficient and functional antigen presenting cells, as well as to assess the type of
allogeneic T-cell response against B-CLL – derived DCs. Material and methods: B-CLL cells from 25 patients were cultured
with or without the presence of CD40L and IL-4 for 96 hours and then cultured in mixed lymphocyte reaction with allogeneic
T-cells. Results: 1) after CD40 stimulation B-CLL cells achieved phenotypical and functional characterization of DCs (i.e.
upregulated co-stimulatory and adhesion molecules at mRNA and protein level) 2) leukemia-derived DCs expressed higher
amount of mRNA for chemokines involved in T-cell migration (MDC, TARC and CCR7) 3) the proliferating response of Tcells
against leukemia-derived DCs consisted of CD4 and CD8 cells (upregulation of HLA-DR and OX40). Conclusions:
our experiment confirm that B-CLL cells can be turned into dendritic-like cells, additionally, these cells express chemokines
involved in T-cell migration and stimulate allogeneic response.
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| Keywords: CD40L; chronic lymphocytic leukemia; dendritic cells; T – lymphocytes |
| Year: 2007, Volume: 54, Issue: |
Page From: 527, Page To: 535 |
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