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CUSTOMERS Acta Virologica 2005 Acta Virologica Vol.49, p.235-241, 2005
CUSTOMERS Acta Virologica 2005 Acta Virologica Vol.49, p.235-241, 2005
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Acta Virologica Vol.49, p.235-241, 2005 |
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| Title: INHIBITION OF HEPATITIS B VIRUS REPLICATION BY RECOMBINANT SMALL INTERFERING RNAs | ||
| Author: K. WU, Y. GONG, X. ZHANG, Y. ZHANG, Y. MU, F. LIU, D. SONG, Y. ZHU, J. WU | ||
| Abstract: RNA interference (RNAi) is a biological phenomenon in which introduction of a small, doublestranded
interfering RNAs (siRNAs) into a cell causes a specific degradation of homologous single-stranded
RNA. siRNA can be delivered into the cell by different approaches including synthetic RNA, in vitro transcribed
RNA and RNA transcribed from polymerase III-based recombinant vectors. As hepatitis B (HB) represents
a worldwide health problem, we attempted to develop a fast and easy approach to generation and screening of
specific siRNA-targeted HB virus (HBV) genes. Using PCR amplification, specific siRNA expression cassettes
(SECs) were developed and used to generate effective siRNAs against HB virus (HBV) replication and gene
expression in mammalian cells. After screening, we identified two SECs that expressed siRNAs which efficiently
decreased the level of HBV pre-c/c gene expression in transfected Bel-7402 cells by 81.9% and 87.3%,
respectively. In addition, the level of HBV DNA was decreased by 83.5% and 85.2% in HepG2 2.2.15 cells,
respectively. This study provides (i) a new effective application of RNA interference to study viral gene function
and viral replication and (ii) a new tool for the prevention and treatment of human HBV infection. |
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| Keywords: Hepatitis B virus; RNA interference; siRNAs; HBV replication; HBV DNA replication | ||
| Year: 2005, Volume: 49, Issue: | Page From: 235, Page To: 241 | |
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