Home Neoplasma 2008 Neoplasma Vol.55, No.2, p.130-137, 2008

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6 times a year.
Founded: 1954
ISSN 0028-2685
ISSN 1338-4317 (online)

Published in English

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Neoplasma Vol.55, No.2, p.130-137, 2008

Title: Long-Term BRCA1 Down-Regulation by Small Hairpin RNAs Targeting the 3’ Untranslated Region

Abstract: Mutations in the BRCA1 gene are responsible for the majority of hereditary breast/ovarian cancers. The functional significance of many mutations/splicing variants identified during the screening of high-risk individuals is difficult to predict due to the lack of in vitro functional tests correlating sequence variants with a risk of cancer development. RNA interference is a promising tool in analyzing functional properties of BRCA1 mutations. Here we designed and functionally analyzed shRNAs directed to 3’-UTR of BRCA1 mRNA that may be used to knock-down expression of endogenous BRCA1. Using retroviral infection, we achieved long-term down-regulation of BRCA1 in a cell-type specific manner. We propose that 3’-UTR-directed shRNAs, coupled with up-regulation of exogenous mutated BRCA1 variants, may constitute a versatile system for the functional analysis of BRCA1 gene alterations.

Keywords: BRCA1, Retroviral infection, RNAi, shRNA, UTR
Year: 2008, Volume: 55, Issue: 2 Page From: 130, Page To: 137

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