Acta Virologica Vol.49, p.261-270, 2005
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Title: ANALYSIS OF SPECIFICITY OF INTERACTION BETWEEN
SYNTHETIC KEY SACCHARIDE COMPONENTS OF
LIPOPOLYSACCHARIDES AND MONOCLONAL ANTIBODIES TO
COXIELLA BURNETII |
Author: E. KOVÁČOVÁ1, D. CMARKO2, V. MUCHA1, R. TOMAN1, F. ČIAMPOR1, P. KOSMA3, J. TYCZKA4, Z. SEKEYOVÁ1* |
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Abstract: Two monoclonal antibodies (MAbs) against the lipopolysaccharides (LPSs) of Coxiella burnetii
(C.b.) strains Priscilla and Nine Mile were prepared characterized by their interaction with synthetic
glycoconjugates representing parts of LPSs of C.b. in virulent phase. Both MAbs were directed against
immunodominant epitopes comprising core constituent of LPSs, Kdo (3-deoxy-α-D-manno-2-octulopyranosylonic
acid). ELISA showed that the anti-Nine Mile MAb 4/11 bound preferably to disaccharides
(α-Kdo (2 → 4) α-Kdo and α-Kdo (2→4) α-(5d) Kdo), while the anti-Priscilla MAb 1/4/H bound to all
conjugates, though with various intensity. On the other hand, immunoelectron microscopy revealed a positive
binding of only one glycoconjugate, namely the trisaccharide α-Kdo (2→4) α-Kdo (2→4) α-Kdo-BSA, to
both MAbs. In competitive ELISA (cELISA), the anti-Priscilla MAb 1/4/H distinguished the strains Nine Mile
and Priscilla, while the anti Nine Mile MAb 4/11 did not.
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Keywords: Coxiella burnetii; monoclonal antibodies; synthetic saccharides; lipopolysaccharides; ELISA; cELISA; immunoelectron microscopy |
Year: 2005, Volume: 49, Issue: |
Page From: 261, Page To: 270 |
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