Home CONTACT Neoplasma 2008 Neoplasma Vol.55, No.6, p.513-518, 2008

Journal info


6 times a year.
Founded: 1954
ISSN 0028-2685
ISSN 1338-4317 (online)

Published in English

Editorial Info
Abstracted and Indexed
Submission Guidelines

Select Journal







Webshop Cart

Your Cart is currently empty.

Info: Your browser does not accept cookies. To put products into your cart and purchase them you need to enable cookies.

Neoplasma Vol.55, No.6, p.513-518, 2008

Title: Nodal status in the papillary thyroid vancer. Comparison of the results of routine histopathological examination, immunohistochemistry and reverse transcription – polymerase chain reaction.
Author: K. KACZKA, M. JAKUBIAK-WIELGANOWICZ, I. ZAWLIK, R. ZIOLKOWSKI, K. KUZDAK, L. POMORSKI

Abstract: Immunohistochemistry (IE) and polymerase chain reaction (PCR) are tools enabling to find small number of tumor cells in lymph nodes (LNs) or peripheral blood. Perhaps these methods will allow early detection of cell dissemination and refine risk group within papillary thyroid cancer (PTC) that might benefit from more extensive surgical procedures or adjuvant therapy. In our study we detected PTC cells in the cervical LNs by routine histopathological examination RHE), IE and RT-PCR and compared obtained results. We also estimated the impact of RT-PCR and IE results on TNM staging and clinical staging according to UICC in patients with PTC. Each of 216 LNs from 28 patients with PTC were divided into two parts: one for RHE and IE the other one for Tg mRNA RT-PCR. Nodal metastases of PTC, in the regional LNs, were found by RT-PCR only in 1(3.6%) patient more than in RHE. In other 4(14.3%) patients molecular examination increased number of involved LNs. In the other patient it revealed less metastasized LNs. The molecular examination changed nodal status in 5(17.9%) of 28 patients. TNM staging was altered from N0 to N1 in one patient. In the others was changed only the number of involved LNs Our research prooved that Tg mRNA RT-PCR technique was sensitive method for detection of nodal metastases of PTC. The outcomes of RT-PCR are similar to RHE so that examination really does not change the estimation of the disease staging according to UICC classification and main surgical therapy in PTC patients.

Keywords: Thyroid cancer, lymph node metastases,histopathological examination immunohistochemistry, reverse transcription-polymerase chain reaction.
Year: 2008, Volume: 55, Issue: 6 Page From: 513, Page To: 518



download file



© AEPress s.r.o
Copyright notice: For any permission to reproduce, archive or otherwise use the documents in the ELiS, please contact AEP.