Home Acta Virologica 2006 Acta Virologica Vol.50, p.39-44, 2006

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Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

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Acta Virologica Vol.50, p.39-44, 2006

Title: GENETIC ANALYSIS OF INDIAN BOVINE VIRAL DIARRHEA VIRUS 1 ISOLATES IN NPRO AND ENTIRE GENE REGION CODING STRUCTURAL PROTEINS
Author: N. MISHRA, Š. VILČEK, P. JAIN, S.S. PITALE, H.K. PRADHAN

Abstract: Three Indian Bovine viral diarrhea virus 1 (BVDV-1) isolates were analyzed at genetic level in Npro (viral autoprotease) and entire gene region coding structural proteins, namely capsid (C) protein, Erns, and envelope proteins E1 and E2. All these isolates were found to be of b subtype based on the entire 504 nt region of Npro and 1119 nt region of E2. However, in comparison with other isolates of this subtype, they were allocated inside the BVDV-1 subtype b cluster to a separate clade with a longer distance. Of six cysteine residues in Npro only three were totally conserved in all three isolates. The isolates showed 94.9–99.3% and 92.2–99.0% identities for the entire C-E2 gene region at nucleotide and amino acid levels, respectively. The lowest identity values (88.5–91.7%) were observed for E2 amino acid sequences. The identity of the isolates with Osloss, a reference BVDV-1 subtype b strain, was in the range of 82.1–89.9% for nucleotide and 78.6–89.2% for amino acid sequences in the C-E2 region. The Npro/C and Erns/E1 cleavage sites were highly conserved. The C/Erns and E1/E2 cleavage sites were more conserved from the N-end of Erns and the C-end of E1, respectively. These findings suggest that some unique mutations have occurred in the described Indian BVDV-1 isolates, though they all belong to the BVDV-1 subtype b.

Keywords: Bovine viral diarrhea virus; pestivirus; autoprotease; structural proteins; genetic analysis; phylogeny
Year: 2006, Volume: 50, Issue: Page From: 39, Page To: 44



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