Home Acta Virologica 2006 Acta Virologica Vol.50, p.93-99, 2006

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Quarterly,
Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

Published in English

Impact Factor = 1.82

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Acta Virologica Vol.50, p.93-99, 2006

Title: INDUCTION OF TUMOR NECROSIS FACTOR ALPHA IN MURINE MACROPHAGES WITH VARIOUS STRAINS OF COXIELLA BURNETII AND THEIR LIPOPOLYSACCHARIDES
Author: M. KUBEŠ, Z. KUZMOVÁ, E. GAJDOŠOVÁ, R. IHNATKO, V. MUCHA, R. TOMAN, E. KOVÁČOVÁ

Abstract: e ability of various strains of Coxiella burnetii (C.b.) and their phase I and II lipopolysaccharides (LPSs) to induce tumor necrosis factor alpha (TNF-α) in peritoneal Balb/c mouse macrophages in vitro was investigated. Considerable differences in the induction ability were observed in dependence on the strain applied. In a TNF-α bioassay, the most effective inducers were both corpuscles and LPSs of the strains Priscilla and Scurry, followed by Nine Mile, Luga, and Henzerling I. In contrast, in ELISA, the most effective inducers were LPSs of the strains Luga and Henzerling, followed by Nine Mile, Priscilla, and Scurry. The role of toll-like receptor 4 (TLR4) in the induction was confirmed by the use of C3H/HeJ mouse macrophages. Thus, the induction of TNF-α was much higher in Balb/c mouse macrophages than that in TLR4-deficient C3H/HeJ mouse macrophages. Differences in the results of the bioassay and those of ELISA suggest a role of another secreted factor(s) induced with C.b. in murine macrophages that could act synergically with TNF-α in L929 cells in the bioassay. The observed differences in TNF-α induction might play a role in the pathobiology of Q fever.

Keywords: TNF-α; macrophage; bioassay; ELISA; Coxiella burnetii; lipopolysaccharides
Year: 2006, Volume: 50, Issue: Page From: 93, Page To: 99



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