Home Acta Virologica 2006 Acta Virologica Vol.50, p.101-105, 2006

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Quarterly,
Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

Published in English

Impact Factor = 1.82

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Acta Virologica Vol.50, p.101-105, 2006

Title: DEVELOPMENT OF A REAL-TIME PCR FOR TOMATO YELLOW LEAF CURL SARDINIA VIRUS
Author: S. GHARSALLAH CHOUCHANE, F. GORSANE, H. LOUZIR, M.K. NAKHLA, M. MARRAKCHI, H. FAKHFAKH

Abstract: Recently, tomato yellow leaf curl disease has become important for the tomato grown both in greenhouse and field conditions in Tunisia. Here, we describe a rapid, specific, reliable, and sensitive real-time PCR, based on TaqMan® chemistry, for Tomato yellow leaf curl Sardinia virus (TYLCSV). This method proved suitable for the detection and quantification of this virus in tomato, pepper and bean plants. It detected the virus even in the samples that were negative by conventional assays.

Keywords: coat-protein gene; real-time PCR; TaqMan® chemistry; Tomato yellow leaf curl Sardinia virus; SYBR® Green I chemistry
Year: 2006, Volume: 50, Issue: Page From: 101, Page To: 105



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