Home Acta Virologica 2008 Acta Virologica Vol.52, No.4, p.243-249, 2008

Journal info


Quarterly,
Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

Published in English

Impact Factor = 1.82

Aims and Scope
Abstracted and Indexed

Select Journal







Webshop Cart

Your Cart is currently empty.

Info: Your browser does not accept cookies. To put products into your cart and purchase them you need to enable cookies.

Acta Virologica Vol.52, No.4, p.243-249, 2008

Title: Immunological properties of a fusion protein containing nucleocapsid protein and glycoprotein Gn of Hantaan virus
Author: W. Luo, F. Zhang, Y. Yan, X. Wu, Y. Liu, W. Bai, Q. Zhao, H. Wang, Z. Xu

Abstract:

Escherichia coli and the baculovirus Bac-to-Bac system were used to express chimeric gene GnS0.7 consisting of glycoprotein Gn gene and the 0.7 kb fragment of S genome segment carrying nucleoprotein (N) gene of Hantaan virus (HTNV). The expressed fusion protein GnN0.7 was recognized by monoclonal antibodies (MAbs) to HTNV Gn glycoprotein and N protein, respectively. Its molecular mass as determined by Western blot analysis corresponded to the predicted value. The mice immunized with insect Spodoptera frugiperda (Sf9) cells transfected with recombinant baculovirus carrying GnS0.7 gene, produced serum antibodies with titer up to 3,200 as assayed by immunofluorescence. Moreover, immunized mice showed positive proliferation index for splenocytes stimulated with HTNV Gn and N, respectively. These results indicated that insect Sf9 cells infected with the recombinant baculovirus expressed a fully biologically active fusion protein that elicited not only humoral but also cellular immune response in mice. Hence, this protein may be used as a genetically engineered subunit HTNV vaccine representing efficacious and safe alternative to traditional vaccines.



Keywords: Hantaan virus; fusion protein; immune response
Year: 2008, Volume: 52, Issue: 4 Page From: 243, Page To: 249

Price: 16.80 €






© AEPress s.r.o
Copyright notice: For any permission to reproduce, archive or otherwise use the documents in the ELiS, please contact AEP.