Home HOME Acta Virologica 2006 Acta Virologica Vol.50, p.107-113, 2006

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Quarterly,
Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

Published in English

Impact Factor = 1.82

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Acta Virologica Vol.50, p.107-113, 2006

Title: MOLECULAR VARIABILITY OF THE COAT PROTEIN GENE OF POTATO VIRUS Y FROM TOBACCO IN CHINA
Author: N. LI, X. WANG, G. ZHOU, J. DONG
Abstract: Thirty-three tobacco samples showing typical symptoms of Potato virus Y (PVY) infection were obtained from tobacco fields in various regions of China. The results of indirect ELISA confirmed the infection with PVY. All the isolates had a capacity to infect tobacco systemically in greenhouse, causing either of two main symptoms: veinal necrosis and mosaic. The nucleotide and amino acid sequences of the coat protein (CP) gene and protein, respectively, of the isolates were determined. Comparison of the isolates revealed a high conservation of the CP gene with an identity of 83.2%. A phylogenetic tree of 41 Chinese isolates of PVY, based on complete CP gene, showed 3 groups corresponding to the strains PVYNTN (A group), PVYo (C group) and a putative new strain similar to PVYN (B group). The amino acid sequences of complete CP protein of the isolates showed an identity of 87.6%. The highest identity was observed in the C-terminal half of the CP protein, where only 11 amino acid differences could be observed, in contrast to the N-terminal half with 22 differences.
Keywords: Potato virus Y; Chinese isolates; coat protein; multiple sequence alignment; phylogenetic tree; tobacco; nucleotide sequencing
Year: 2006, Volume: 50, Issue: Page From: 107, Page To: 113
Price: 16.80 €





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