Acta Virologica Vol.50, p.135-138, 2006

Quarterly,
Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

Published in English

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Acta Virologica Vol.50, p.135-138, 2006
Title: DETECTION OF TRANSGENE COPY NUMBER BY ANALYSIS OF THE T1 GENERATION OF TOBACCO PLANTS WITH INTRODUCED P3 GENE OF POTATO VIRUS A
Author: Z. ŠUBR1, S. NOVÁKOVÁ1, H. DRAHOVSKÁ2

Abstract: Real-time PCR, namely the ΔΔCt method was used to determine the relative copy number of Potato virus A (PVA) P3 gene in the genome of the T1 generation of 18 transgenic tobacco lines. These results were compared with segregation ratios of kanamycin (Km)-resistant phenotype in T1 plants of each line and were found to be, in general, concordant. All the five lines with the Mendelian segregation ratio of 3:1 carried one gene copy. In 12 of 13 lines with uneven segregation more inserted gene copies were detected. Only for one line the real-time PCR and phenotype segregation differed. According to our results the real-time PCR of T1 generation may be used as supplementary method of estimation of the number of transgene copies in the case of nonavailability of the original T0 plants.

Keywords: real-time PCR; transgenic tobacco; phenotypic segregation; Potato virus A; potyvirus
Year: 2006, Volume: 50, Issue:	Page From: 135, Page To: 138


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