Home Acta Virologica 2006 Acta Virologica Vol.50, p.181-186, 2006

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E-ISSN 1336-2305

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Acta Virologica Vol.50, p.181-186, 2006

Title: EVALUATION OF CLINICAL SPECIMENS FOR INFLUENZA A VIRUS POSITIVITY USING VARIOUS DIAGNOSTIC METHODS
Author: E. VAREČKOVÁ, H. BLAŠKOVIČOVÁ, M. GOCNÍK, J. MIKAS, J. ADAMČÁKOVÁ, T. FISLOVÁ, F. KOSTOLANSKÝ, V. MUCHA

Abstract: The diagnostic method for Influenza A virus, utilizing the SERION ELISA Antigen kit (SERION EIA), if results were evaluated according to the manufacturer's instructions, has repeatedly failed to detect a great number of clinical samples positive by virus isolation and RT-PCR. Therefore we compared the SERION EIA with the one-step 44/107L-Px immunocapture enzyme immunoassay (44/107L-Px EIA), developed in our laboratory (Tkáčová and Varečková, J. Virol. Methods 60, 65–71, 1996). Seventy-three clinical specimens, of which 65 were positive by virus isolation (used as reference method), were tested by both EIAs. By the SERION EIA, out of the 65 reference-positive samples only 8 (12%) were positive, 5 (8%) were ambiguous, and 52 (80%) were negative, which corresponded to the sensitivity of 12%. On the contrary, the sensitivity of the 44/107L-Px EIA was 74%. However, the calculation of cut-off values for the evaluation of positivity of clinical specimens in these two assays were not the same. If the evaluation procedure used for the 44/107L-Px EIA was applied to the SERION EIA, the sensitivity and the specificity of both EIAs became comparable, namely 71% and 100% for the SERION EIA and 74% and 100% for the 44/107L-Px EIA, respectively. From these results it follows that not the detection ability of the SERION EIA, but the evaluation procedure recommended by its manufacturer led to a loss of large number of positive specimens.

Keywords: Influenza A virus; clinical specimens; diagnostics; enzyme immunoassay; SERION ELISA Antigen kit; sensitivity, specificity; virus isolation; RT-PCR
Year: 2006, Volume: 50, Issue: Page From: 181, Page To: 186

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