Home HOME Acta Virologica 2006 Acta Virologica Vol.50, p.195-200, 2006

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Quarterly,
Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

Published in English

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Acta Virologica Vol.50, p.195-200, 2006

Title: CLONING, EXPRESSION AND IDENTIFICATION OF THE GENE OF HUMAN SINGLE-CHAIN VARIABLE FRAGMENT ANTIBODY AGAINST HEPATITIS B VIRUS SURFACE ANTIGEN
Author: Y.-Z. LUN, J. CHENG, Y.-W. ZHONG, B.-C. ZHAO

Abstract: Expression of single-chain variable fragment (scFv) antibodies on the surface of bacteriophage is widely used to prepare antibodies with pre-defined specificities. A phage antibody library containing the gene for scFv antibody against Hepatitis B virus surface antigen (HBsAg) was panned with HBsAg immobilized on microtiter plate wells. After five rounds of panning 30 phage clones specific to HBsAg were obtained and one selected clone was sequenced. It was found to consist of 789 bp and its amino acid sequence and specifically detected the respective antigen in the patients but not in healthy persons.

Keywords: Hepatitis B virus surface antigen; phage display; single-chain variable fragment antibody
Year: 2006, Volume: 50, Issue: Page From: 195, Page To: 200

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