Home Acta Virologica 2006 Acta Virologica Vol.50, p.201-205, 2006

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Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

Published in English

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Acta Virologica Vol.50, p.201-205, 2006

Title: PARTIAL MOLECULAR CHARACTERIZATION OF A MILD ISOLATE OF GRAPEVINE FANLEAF VIRUS FROM SOUTH MORAVIA, CZECH REPUBLIC
Author: P. KOMÍNEK, M. BRYXIOVÁ, M. GLASA

Abstract: An atypical mild isolate HV5 of Grapevine fanleaf virus (GFLV) was found in a South Moravian viticulture region in Czech Republic. Partial sequence of its RNA2 was determined and compared with available sequences of typical GFLV isolates. Two genomic regions, namely a 814 nt-long one spanning the movement protein (MP) gene and a 5'-part of the coat protein (CP) gene, and a 1426 nt-long one covering a part of the CP gene and the adjacent 3'-non-coding region (3'-NCR) were analyzed. Although no HV5-specific molecular features could be found in the two regions, marked differences were observed in the 3'-NCR. There was a 54 nt-long portion in which the sequence identity of some compared isolates was only 54.7%. Moreover, an unique one-nucleotide deletion occurred in the HV5 3'-NCR. These changes were also reflected in the predicted RNA secondary structure of this region. Particular biological behavior of GFLV HV5 isolate, namely a symptomless infection, could be related to the observed molecular differences.

Keywords: Grapevine fanleaf virus; movement protein; coat protein; secondary RNA structure
Year: 2006, Volume: 50, Issue: Page From: 201, Page To: 205

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