Home HOME Acta Virologica 2006 Acta Virologica Vol.50, p.217-222, 2006

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Quarterly,
Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

Published in English

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Acta Virologica Vol.50, p.217-222, 2006

Title: THE M AND N GENES-BASED SIMPLEX AND MULTIPLEX PCRs ARE BETTER THAN THE F OR H GENE-BASED SIMPLEX PCR FOR PESTEDES- PETITS-RUMINANTS VIRUS
Author: A. GEORGE, P. DHAR, B.P. SREENIVASA, R.P. SINGH, S.K. BANDYOPADHYAY

Abstract: Summary. – Nucleocapsid (N), matrix (M) and hemagglutinin (H) genes-based simplex PCRs and an N and M genes-based multiplex PCR were developed for detection of Peste-des-petits-ruminants virus (PPRV). The M gene PCR was the most sensitive, followed by N, H and an already described fusion (F) gene PCRs, as they could detect the virus in samples with titers of 101, 102, 104and 105 TCID50/ml, respectively. The multiplex PCR was as sensitive as the M gene PCR, but it had the advantage of differentiating PPRV from Rinderpest virus (RPV).

Keywords: Peste-des-petits-ruminants virus; PCR; RT-PCR; M gene; N gene; H gene; F gene; multiplex PCR
Year: 2006, Volume: 50, Issue: Page From: 217, Page To: 222

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