General Physiology and Biophysics Vol.27, p.253-262, 2008

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ISSN 1338-4325 (online)
ISSN 0231-5882 (print)
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General Physiology and Biophysics Vol.27, p.253-262, 2008
Title: Characterization and regulation of basal calcium influx in human peripheral blood lymphocytes
Author: Boris Lakatoš, Jana Slováková, Karin Kaiserová, Jozef Orlický and Ľudovít Varečka

Abstract: The basal 45Ca2+ influx into resting human blood lymphocytes was measured. This process showed biphasic kinetics with first rapid phase followed by the second long-lasting and markedly slower phase. Further, it showed signs of saturability and reaches maximal values at 37°C and extracellular pH 7.2. The basal 45Ca2+ influx was stimulated by addition of submicromolar concentrations of 4β-phorbol-12-myristate-13-acetate, and this effect was abolished by protein kinase C (PKC) inhibitor Ro-31-8220. In the regulation of basal 45Ca2+ influx is probably only partially involved adenylate cyclase pathway as show results with intracellular c-AMP elevating agents (dB-c-AMP, 3-isobutyl-1-metylxantine and forskolin). Uncoupler 3,3´,4´,5-tetrachloro-salicylanilide (TCS) in micromolar concentrations stimulated basal 45Ca2+ influx and its effect was more significant in media with high extracellular concentration of K+.

Keywords: Lymphocytes — Calcium influx — PKC — Amiloride
Year: 2008, Volume: 27, Issue: 4	Page From: 253, Page To: 262


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