Home CUSTOMERS Acta Virologica 2007 Acta Virologica Vol.51, p.51-54, 2007

Journal info


Quarterly,
Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

Published in English

Impact Factor = 1.82

Aims and Scope
Abstracted and Indexed

Select Journal







Webshop Cart

Your Cart is currently empty.

Info: Your browser does not accept cookies. To put products into your cart and purchase them you need to enable cookies.

Acta Virologica Vol.51, p.51-54, 2007

Title: A NOVEL DOUBLE-STABLE T-REX/GB CELL LINE EXPRESSING GLYCOPROTEIN B OF HERPES SIMPLEX VIRUS 1
Author: R. TANGELMAYER, V. DURMANOVA, C.W. KNOPF, M. KUDELOVA, I. REZUCHOVA

Abstract: We established and characterized a new stable T-REx/gB cell line expressing gB of HSV-1 under tetracycline (Tet) control. The expression of complete gB (120 K) in T-REx/gB cells was detected by Western blot analysis with anti-HSV-1/ANG/gB monoclonal antibody as early as 2 days after Tet induction. Inducibility and tightness of Tet-regulated gB expression in T-REx/gB cell line was shown to be preserved after long-term culture (2 months) and after numerous freezing/thawing cycles as well. In this study, we described the conditions required for the generation of the T-REx/gB cell line, which can be useful as a host for the isolation and propagation of HSV-1 recombinant viruses defective in gB gene.

Keywords: cell line expressing gB; Herpes simplex virus 1; inducible expression
Year: 2007, Volume: 51, Issue: Page From: 51, Page To: 54

Price: 9.60 €






© AEPress s.r.o
Copyright notice: For any permission to reproduce, archive or otherwise use the documents in the ELiS, please contact AEP.