Home CONTACT Acta Virologica 2003 Acta Virologica Vol.47, p.97-104, 2003

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Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

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Acta Virologica Vol.47, p.97-104, 2003

Title: SEQUENCE AND PHYLOGENETIC ANALYSIS OF SH, G, AND F GENES AND PROTEINS OF HUMAN RESPIRATORY SYNCYTIAL VIRUS ISOLATES FROM SINGAPORE
Author: C.S. LIM, G. KUMARASINGHE, V.T.K. CHOW

Abstract: To study the genetic variability and molecular epidemiology of Human respiratory syncytial virus (HRSV) occurring in Singapore, nucleotide sequencing of three membrane-associated genes (SH, G and F) of four local isolates was performed. Comparison of their nucleotide and amino acid sequences with those of the prototype strains A2 (subgroup A) and CH-18537 (subgroup B) indicated that the Singapore isolates belong to the subgroup A. Comparison of the Singapore isolates with the reference strain A2 showed that whereas the G protein was the most divergent with up to 15% difference, the F and SH proteins showed less diversity of only up to 4%. Each gene exhibited its distinct variable and conserved regions. The N- and O-glycosylation sites within the G protein of the isolates were analyzed to ascertain their potential implications on the antigenicity of the viral glycoprotein. Based on the second variable region of the G protein, phylogenetic analysis of the Singapore isolates with 91 previously identified genotypes of subgroup A revealed that more than one genotype (GA2 and GA5) may circulate in the local population at a given time. This epidemiological study reflects the pattern of genetic relationships between the HRSV isolates from Singapore to those from other parts of the world.

Keywords: F, G, SH genes/proteins; genotypes; phylogeny; Human respiratory syncytial virus; sequence analysis
Year: 2003, Volume: 47, Issue: Page From: 97, Page To: 104



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