Home Acta Virologica 2007 Acta Virologica Vol.51, p.135-138, 2007

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Quarterly, 72-88 pp. per issue
Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

Published in English

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Acta Virologica Vol.51, p.135-138, 2007

Title: ELECTROPHORETIC MOBILITY OF THE CAPSID PROTEIN OF THE PLUM POX VIRUS STRAIN PPV-REC INDICATES ITS PARTIAL PHOSPHORYLATION
Author: Z. SUBR, H. RYSLAVA, E. KOLLEROVA

Abstract: A double-band SDS-PAGE profile was found reproducible for capsid protein (CP) of Plum pox virus (PPV) isolates belonging to the strain PPV-Rec. The double-band was also present in the virus population multiplied in various plants. A single-lesion passage in a hypersensitive host Chenopodium foetidum showed that its presence was not a result of a mixed infection. We found that the two electrophoretic forms of CP shared identical N-terminus. Therefore, they did not originate from an alternative proteolytic processing, but were different in their posttranslational modification. The slower band of CP could be converted to the faster one by the phosphatase treatment. We assumed that CP protein was present in both phosphorylated and dephosphorylated forms in the infected plants.

Keywords: electrophoretic mobility; phosphorylation; potyvirus
Year: 2007, Volume: 51, Issue: Page From: 135, Page To: 138



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