Home Acta Virologica 2007 Acta Virologica Vol.51, p.149-156, 2007

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Quarterly, 72-88 pp. per issue
Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

Published in English

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Acta Virologica Vol.51, p.149-156, 2007

Title: SPIKE PROTEIN REGION (aa 636–789) OF PORCINE EPIDEMIC DIARRHEA VIRUS IS ESSENTIAL FOR INDUCTION OF NEUTRALIZING ANTIBODIES
Author: D.B. SUN, L. FENG, H.Y. SHI, J.F. CHEN, S.W. LIU, H.Y. CHEN, Y.F. WANG

Abstract: To analyze antigenic structure of the spike (S) protein of Porcine epidemic diarrhea virus (PEDV), the gene encoding its major immunodominant region S1 was amplified by PCR. We prepared four truncated S1 proteins spanning the entire S1 domain fused to GST protein. To identify the most important antigenic region of S1, the truncated S1-GST fusion proteins were examined for their ability to react with immune serum against PEDV and to elicit the formation of neutralization antibodies in immunized animals. We found that the region of S1 signed as S1D (aa 636–789) was able to react with PEDV antiserum and to elicit formation of neutralization antibodies in mice. Moreover, the immune serum against S1D showed the binding ability to the native S protein of PEDV.

Keywords: epitope; Porcine epidemic diarrhea virus; spike protein; virus neutralization
Year: 2007, Volume: 51, Issue: Page From: 149, Page To: 156



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