Home CONTACT Acta Virologica 2003 Acta Virologica Vol.47, p.105-111, 2003

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Quarterly,
Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

Published in English

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Acta Virologica Vol.47, p.105-111, 2003

Title: MOLECULAR CHARACTERIZATION OF A CARNATION ETCHED RING VIRUS ISOLATE FROM INDIA
Author: G. Raikhy, V. Hallan, S. Kulshrestha, M. Lal Sharma, R. Ram, A.A. Zaidi

Abstract: Incidence of the Carnation etched ring virus (CERV), the only DNA virus reported to date on carnation, was investigated by a bioassay using a partially purified virus as inoculum and then by a double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA). Out of 61 carnation cultivars analyzed 41 (67%) were found positive. The virus positivity was verified by polymerase chain reaction (PCR) and nucleotide sequencing. The amplified 1349 bp fragment was by about 98% and 96% identical with respect to coat protein (CP) and enzymatic polyprotein genes, respectively, as compared to the sequences available in the database. In terms of amino acid sequence similarity, the homology values were 99% and 97%, respectively. Comparison with other caulimoviruses revealed that CERV is most closely related to the Cauliflower mosaic virus (CaMV). High genetic stability of CERV may be attributed to the fact that it has evolved from the same initial sequence in an original host. Because of global market of cut flowers and vegetative propagation it has been dispersed around the world.

Keywords: carnation; Carnation etched ring virus; coat protein; enzymatic polyprotein; Indian isolate; nucleotide sequencing
Year: 2003, Volume: 47, Issue: Page From: 105, Page To: 111



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