Home CONTACT Acta Virologica 2007 Acta Virologica Vol.51, p.157-162, 2007

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Quarterly, 72-88 pp. per issue
Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

Published in English

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Acta Virologica Vol.51, p.157-162, 2007

Title: EXPRESSION OF THE BARLEY YELLOW DWARF VIRUS-GAV MOVEMENT PROTEIN AND ITS DETECTION IN THE INFECTED AND TRANSGENIC PLANTS
Author: J. XIE, X. WANG, Y. LIU, Y. PENG, G. ZHOU

Abstract: Movement proteins (MPs) that facilitate virus movement in the plants were identified in a number of plant viruses. In this study, full-length MP gene of the Chinese isolate Barley yellow dwarf virus-GAV (BYDV-GAV) was cloned and expressed in Escherichia coli. About 32% of the expressed MP was soluble providing the concentration of isopropyl-ß-D-galactopyranoside (IPTG), time of the induction, temperature and shaking speed were optimized. The soluble MP was purified using nickel-affinity column. Immune serum prepared against purified MP was used for the detection of MP in the BYDV-GAV infected leaves of oat and in the leaves of transgenic wheat plants expressing the full-length and truncated MP gene.

Keywords: Barley yellow dwarf virus-GAV; movement protein; expression; immunization
Year: 2007, Volume: 51, Issue: Page From: 157, Page To: 162

Price: 10.80 €






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