Home Acta Virologica 2007 Acta Virologica Vol.51, p.195-201, 2007

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Quarterly, 72-88 pp. per issue
Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

Published in English

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Acta Virologica Vol.51, p.195-201, 2007

Title: INHIBITION OF EXPRESSION OF RNA POLYMERASE WITH SMALL INTERFERING RNAs TARGETING A CONSERVED MOTIF IN THE RESPECTIVE VIRAL GENES IN VIRUSES OF THE FAMILY FLAVIVIRIDAE
Author: Z. XU, X. LI, R. LIU, Y. SI, M. SUN, M. JIN, H. CHEN, P. QIAN

Abstract: The RNA-dependent RNA polymerase (RdRp) in viruses of the family Flaviviridae plays an important role in the viral replication process and in the forming of a replicase complex. We used small interfering RNAs (siRNAs) corresponding to the highly conservative Motif V of RdRp gene of different viruses to examine their role in modulating the expression of RdRp. Evaluation of the expression of RdRps was performed by the fluorescence, flow cytometry, Western blotting, and real-time PCR. We found that Classical swine fever virus (CSFV) siRNA could completely block the transcription and expression of RdRp. Additionally, Hepatitis C virus (HCV) siRNA could cause effective inhibition of RdRp, whereas Japanese encephalitis virus siRNA did not show significant repression of corresponding RdRp. These results demonstrated that siRNAs inhibited the expression of tested RdRps at the transcription level or at the posttranscriptional processing to a different extent.

Keywords: Classical swine fever virus; Japanese encephalitis virus; Hepatitis C virus; small interfering RNAs; RNA-dependent RNA polymerase
Year: 2007, Volume: 51, Issue: Page From: 195, Page To: 201



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