Home General Physiology and Biophysics 2010 General Physiology and Biophysics Vol.29, No.1, p.113–121, 2010

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Founded: 1982
ISSN  1338-4325 (online)

Published in English

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General Physiology and Biophysics Vol.29, No.1, p.113–121, 2010

Title: Effects of one-day reperfusion after transient forebrain ischemia on circulatory system in the rat
Author: Petra Kravcukova, Viera Danielisova, Miroslava Nemethova, Jozef Burda, Miroslav Gottlieb
Abstract:  Although ischemia/reperfusion injury remains incompletely understood, it appears that reactive oxygen species produced mainly during postischemic recirculation play a critical role. The present study examined the impact of forebrain ischemia and subsequent one-day reperfusion on several blood parameters. We determined glutamate concentration in whole blood, measured Cu/Zn- and Mn-SOD (superoxide dismutase) activity in blood cells as well as plasma, and investigated the prevalence of single and double strand breaks of lymphocyte DNA. The results of our experiment showed that the concentration of glutamic acid in whole blood was increased by about 25%. Antioxidant activity of total SOD and Cu/Zn-SOD was reduced in blood cells and plasma. Mn-SOD activity in blood cells was not affected by ischemic insult and one-day reperfusion, but we detected its significantly lower activity in samples of plasma. We observed a weakly reduced level of double and a significantly elevated level of single strand breaks of lymphocyte DNA.
In conclusion, one day of recovery after the ischemic attack failed to return peripheral circulatory system to physiological conditions. Reduced antioxidant capacity in the blood and an elevated level of excitotoxic amino acid glutamate may cause lymphocyte DNA damage, and probably contribute to insufficient postischemic recovery of brain tissue.
Keywords: BSA, bovine serum albumin; DSBs, double strand breaks; EDTA, ethylenediaminetetraacetic acid; NBT, p-nitrotetrazolium blau grade III; PBS, phosphate buffered saline; ROS, reactive oxygen species; SCGE, single cell gel electrophoresis; SHC, sham control sample; SOD, superoxide dismutase; SSBs, single strand breaks; TAE, tris-acetate-EDTA buffer; U, unit of enzyme activity.
Year: 2010, Volume: 29, Issue: 2 Page From: 113, Page To: 121
doi:10.4149/gpb_2010_02_113


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