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General Physiology and Biophysics 2010 General Physiology and Biophysics Vol.29, No.3, p.266–274, 2010
General Physiology and Biophysics 2010 General Physiology and Biophysics Vol.29, No.3, p.266–274, 2010
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General Physiology and Biophysics Vol.29, No.3, p.266–274, 2010 |
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| Title: Influence of sub-chronic diabetes mellitus on functional properties of renal Na+,K+-ATPase in both genders of rats | ||
| Author: Veronika Javorková, Lucia Mézešová, Jana Vlkovičová, Norbert Vrbjar | ||
| Abstract: For characterization of Na+,K+-ATPase, a key enzyme involved in maintenance of intracellular sodium homeostasis, expression of α1 subunit and the ATP- and Na+-binding properties were investigated by Western blot analysis and by enzyme kinetics, respectively. Previous studies documented time-dependent alteration of properties of renal Na+,K+-ATPase from its mobilization after 8 days to serious deteriorations after 16 weeks of diabetes in rats. Characterizing the critical period during development of the disease, when mobilization of Na+,K+-ATPase observed in the acute phase turns to its damage, we examined the enzyme properties after 8 weeks lasting diabetes which was induced by a single intraperitoneal administration of streptozotocin in a dose of 65 mg·kg–1. The unchanged expression of Na+,K+-ATPase α1-subunit in both genders indicates that 8 weeks represent the time when the mobilization of enzyme synthesis observed previously in acute diabetes is lost. In this time the renal Na+,K+-ATPase undergoes structural changes in the vicinity of Na+-binding site resulting in worsened affinity to sodium in both genders as indicated by 13% and 18% increase of KNa value in female and male rats, respectively. However, gender specific was the diabetes-induced decrease in affinity to ATP by 18% which occurred in female rats only. |
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| Keywords: Sodium pump — Kidney — Streptozotocine — Sex difference — α1 subunit | ||
| Year: 2010, Volume: 29, Issue: 3 | Page From: 266, Page To: 274 | |
| doi:10.4149/gpb_2010_03_266 |
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