Acta Virologica Vol.52, No.1, p.47-52, 2008

Quarterly,
Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

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Acta Virologica Vol.52, No.1, p.47-52, 2008
Title: INHIBITION OF REVERSE TRANSCRIPTASE ACTIVITY OF HEPATITIS B VIRUS POLYMERASE BY ß-L-D4A-TP
Author: X. Y. WANG, L. L. GAO, Z. H. HU, H. L. WANG, F. DENG, J. S. LIN

Abstract: ß-L-enantiomer of 2',3'-didehydro-2',3'-dideoxyadenosine-5'-triphosphate (ß-L-D4A-TP) has previously been proven to inhibit the replication of viral DNA in the Hep G2 2.2.15 cells and in transgenic mouse harboring 1.3-fold-overlength genome of Hepatitis B virus (HBV). To study the inhibition mechanism of the nucleoside analog ß-L-D4A-TP, a polymerase reaction in vitro with the recombinant HBV nucleocapsids was conducted to determine the exact mode of inhibition of the HBV replication by ß-L-D4A-TP. The HBV viral DNA and viral DNA-polymerase complex formed in the polymerase reaction were assayed. The results of this study showed that ß-L-D4A-TP inhibited the replication of HBV DNA by inactivating the reverse transcriptase (RT) activity in a concentration-dependent manner. The kinetics of ß-L-D4A-TP inhibition of the RT activity was the result of an apparent competitive inhibition with dATP.

Keywords: Hepatitis B virus; polymerase; reverse transcriptase; ß-L-D4A-TP
Year: 2008, Volume: 52, Issue: 1	Page From: 47, Page To: 52


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