Acta Virologica Vol.52, No.3, p.143-149, 2008

Quarterly,
Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

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Acta Virologica Vol.52, No.3, p.143-149, 2008
Title: EVALUATION OF TWO GENE-SILENCING CONSTRUCTS FOR RESISTANCE TO TOMATO YELLOW LEAF CURL VIRUSES IN NICOTIANA BENTHAMIANA PLANTS
Author: S. GHARSALLAH_CHOUCHANE, F. GORSANE, M.K. NAKHLA, M. SALUS, C.T. MARTIN, D.P. MAXWELL, M. MARRAKCHI, H. FAKHFAKH

Abstract: Infiltration of Agrobacterium tumefaciens into intact plant leaves of N. benthamiana was used to test the efficiency of two virus-based silencing constructs conferring resistance to the closely related begomoviruses. The constructs contained the most conserved sequences of the coat protein (CP) gene and replication-associated protein (Rep) gene of Tomato yellow leaf curl Sardinia virus (Sicily strain) (TYLCSV- [Sic]). Both constructs formed a hairpin structure that enhanced the post-transcriptional gene-silencing mechanism. When agro-infiltrated plants were challenged separately with infectious viruses TYLCSV-[Sic] and Tomato yellow leaf curl virus (TYLCV), the plants showed resistance to TYLCSV-[Sic], but not to the related TYLCV.

Keywords: agro-infiltration; Nicotiana benthamiana; gene silencing; coat protein gene; Rep gene; begomoviruses
Year: 2008, Volume: 52, Issue: 3	Page From: 143, Page To: 149


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