Acta Virologica Vol.47, p.179-184, 2003

Quarterly,
Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

Published in English

Impact Factor = 1.82

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Acta Virologica Vol.47, p.179-184, 2003
Title: DETECTION OF EGG DROP SYNDROME 1976 VIRUS BY POLYMERASE CHAIN REACTION AND STUDY OF ITS PERSISTENCE IN EXPERIMENTALLY INFECTED LAYER BIRDS
Author: N.S. KUMAR, J.M. KATARIA, M. KOTI, K. DHAMA, R. TOROGHI

Abstract: Polymerase chain reaction (PCR) assay was developed for the detection of Egg drop syndrome 1976 (EDS-76) virus in tissues, namely in the uterus, spleen and buffy coat. It was also used to study the persistence of the virus in tissues of experimentally infected layer birds. The PCR assay could detect as little as 10 fg of purified EDS-76 viral DNA. It also amplified the DNA of Fowl adenovirus serotypes 4 (FAV-4) and 8 (FAV-8). The virus persisted in the uterus up to day 21 post infection (p.i.). Detection of EDS-76 viral DNA in the buffy coat could be useful for studying the occurrence of the respective disease in layer bird flocks.

Keywords: egg drop syndrome; Egg drop syndrome 1976 virus; polymerase chain reaction; diagnosis; buffy coat; persistence
Year: 2003, Volume: 47, Issue:	Page From: 179, Page To: 184


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