Home Acta Virologica 2003 Acta Virologica Vol.47, p.237-243, 2003

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Quarterly, 72-88 pp. per issue
Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

Published in English

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Acta Virologica Vol.47, p.237-243, 2003

Title: A RECOMBINANT FUSION PROTEIN AND DNA VACCINES AGAINST FOOT-AND-MOUTH DISEASE VIRUS TYPE ASIA 1 INFECTION IN GUINEA PIGS
Author: S. BOPEGAMAGE, M. BORSANYIOVÁ, A. VARGOVÁ, A. PETROVIČOVÁ, M. BENKOVIČOVÁ, P. GOMOLČÁK

Abstract: On the basis of amino acid (aa) sequence of the tandem repeat 133–158~20–34~133–158 which consisted of aa 133–158 of VP1 and aa 20–34 of VP4 of Foot-and-mouth disease virus (FMDV) type Asia 1 a recombinant prokaryotic expression vector pAS1-P encoding a fusion protein and eukaryotic expression vectors pAS1-E and pAS1-EDCpG-ODN representing DNA vaccines were constructed. Guinea pigs immunized with these vaccines showed both neutralizing antibody and T cell proliferation responses. FMDV challenge tests for the first time showed that the recombinant fusion protein and pAS1-E and pAS1-EDCpG-ODN vaccines protected 86%, 60% and 43% of guinea pigs from FMDV type Asia1 challenge, respectively. The results also indicated that the immune response of animals treated with the vector pAS1-E containing an oligodeoxynucleotide (ODN), which consisted of immunostimulatory cytosine-phosphate-guanosine (CpG) motifs, was augmented by CpG ODN.

Keywords: Foot-and-mouth disease virus; type Asia 1; DNA vaccine
Year: 2003, Volume: 47, Issue: Page From: 237, Page To: 243



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