Home Acta Virologica 2004 Acta Virologica Vol.48, p.15-21, 2004

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Quarterly,
Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

Published in English

Impact Factor = 1.82

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Acta Virologica Vol.48, p.15-21, 2004

Title: A VERO CELL-ATTENUATED GOATPOX VIRUS PROVIDES PROTECTION AGAINST VIRULENT VIRUS CHALLENGE
Author: M. Hosamani, S. Nandi, B. Mondal, R.K. Singh, T.J. Rasool, S.K. Bandyopadhyay

Abstract: An Indian isolate of Goatpox virus (GTPV) was adapted and propagated in Vero cells for development of an attenuated virus. The virus was initially passaged in primary lamb testes cells and subsequently in Vero cells. At the 55th passage, the virus showed evidence of attenuation when tested for safety in sero-negative goats. At this stage, the virus was found to be completely non-pathogenic. The virus was passaged further and the 60th passage was usedfor testing its immunogenicity in goats. The latter were inoculated with 10, 100 and 1000 TCID50 of the attenuated virus by intradermal (i.d.) route and challenged after 28 days with virulent GTPV. The attenuated virus produced no adverse reaction even at the highest dose and conferred complete protection even at the lowest dose against challenge with a high dose (2 x 106 of 50% skin-reactive dose SRD50) of virulent virus. Increased levels of virus-specific serum antibodies could be demonstrated by both indirect enzyme-linked immunosorbent assay (ELISA) and virus neutralization (VN) test in all the immunized goats. No horizontal transmission of the virus from the immunized to in-contact animals took place. Our results suggest that this attenuated virus could be a safe, immunogenic and potent candidate for developing a vaccine against goatpox.

Keywords: attenuated Goatpox virus; immunogenicity; Vero cells
Year: 2004, Volume: 48, Issue: Page From: 15, Page To: 21



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