Home Acta Virologica 2004 Acta Virologica Vol.48, p.29-34, 2004

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Quarterly,
Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

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Impact Factor = 1.82

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Acta Virologica Vol.48, p.29-34, 2004

Title: INITIAL PEPTIDE MASS FINGERPRINTING ANALYSIS OF PROTEINS OBTAINED BY LYSIS OF COXIELLA BURNETII CELLS
Author: L. Skultéty, L. Hernychová, R. Toman, M. Kroča, J. Stulík, A. Macela

Abstract: Whereas the complete genome of Coxiella burnetii (C.b.), the etiological agent of Q-fever, has recently been published (Seshadri et al., Proc. Natl. Acad. Sci. USA. 100, 5455–5460, 2003), the C.b. proteome is still under study. Using the bioinformatic approach, we found in total 309 proteins on two dimensional electroctrophoretic images of C.b. whole cell lysates. Eighteen major protein species were subjected to peptide mass fingerprinting and identified as the products of 6 known open reading frames (ORFs): the chaperone DnaK (heat shock 70 K protein), chaperonin 60 K (GroEL protein, heat shock protein B), DnaJ-like protein djlA (mucoidy activation protein mucZ), elongation factor Ts (EF-Ts), ribosomal protein L7/L12, and chaperonin 10 K (GroES protein, heat shock protein A).

Keywords: Coxiella burnetii; proteins; peptide mass fingerprinting; 2-D electrophoresis; MALDI-ToF
Year: 2004, Volume: 48, Issue: Page From: 29, Page To: 34



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