Home Endocrine Regulations 2003 Endocrine Regulations Vol.37, p.137-144, 2003

Journal info

Quarterly, 50 pp. per issue 
Founded: 1967
ISSN 1210-0668
E-ISSN 1336-0329

Published in English

Aims and Scope
Editorial Info
Submission Guidelines

Select Journal

Webshop Cart

Your Cart is currently empty.

Info: Your browser does not accept cookies. To put products into your cart and purchase them you need to enable cookies.

Endocrine Regulations Vol.37, p.137-144, 2003

Author: R. Groubet, V. Pallet, B. Delage , A. Redonnet, P. Higueret, P. Cassand

Abstract: Objective. Dietary factors can be associated with colorectal cancer. Fatty acids modulate gene expression in various tissues, mediated by activation of the peroxisome proliferator activated receptor: PPAR. Vitamin A signalling is mediated by retinoic acid (RA) receptors (RAR) and retinoid X receptors (RXR). The steroid nuclear receptors PPAR, RAR, RXR, are DNA-binding proteins and they induce gene transcription upon activation by specific ligands and interacting with distinct promoter sequences in the target genes. The aim of this study was to investigate the impact of hyperlipidic diets on the expression of PPARg, RXRa and RARb mRNA in rat colon. Methods. Rats were fed during 4 weeks with the following diets: a cafeteria diet where 60 % of the energy was supplied as lipids and a high fat diet (HFD) represented by 25 % of a safflower oil (w/w) rich in polyunsaturated fatty acids, mainly n-6. Nuclear receptors mRNA were quantified by real-time RT-PCR with TaqMan probe process or SYBRGreen I chemical. Results. The cafeteria diet and the HFD induced a significant decrease in RARb mRNA: -36 % (p<0.02) and –64 % (P<0.001) respectively. Simultaneously, an increased expression of PPARg mRNA was observed for cafeteria diet +35% (P<0.05) and for HFD +45 % (P<0.05). The level of RXRa mRNA was significantly increased for cafeteria diet: +53% (P<0.0002), while no significant difference in RXRa mRNA was observed in colonic mucosa rats whose fed with the 25 % HFD. Conclusions. These results showed that an hyperlipidic diet could induce early modifications in the pattern of expression of nuclear receptors in rat colon. Many mechanisms could be probably involved but one hypothesis is that a modification of the balance between the nuclear receptors, resulting from an increased expression of PPARg, could induce a decreased expression of RARb in rat colon.

Keywords: Rat colon – Real-time RT-PCR – High polyunsaturated fatty acid diet – Nuclear receptors expression
Year: 2003, Volume: 37, Issue: Page From: 137, Page To: 144

Price: 14.40 €

© AEPress s.r.o
Copyright notice: For any permission to reproduce, archive or otherwise use the documents in the ELiS, please contact AEP.