Acta Virologica Vol.48, p.85-89, 2004

Quarterly,
Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

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Acta Virologica Vol.48, p.85-89, 2004
Title: GENETIC DIVERSITY OF CHICKEN ANEMIA VIRUS FOLLOWING CELL CULTURE PASSAGING IN MSB-1 CELLS
Author: M.S. HASMAH, A.R. OMAR, K.F. WAN, M. HAIR-BEJO, I. AINI

Abstract: It has been shown that a chicken anemia virus (CAV) isolates which had undergone 60 passages in MSB-1 cells (SMSC-1/P60, 3-1/P60) acquired 33–66 nucleotide substitutions at the coding region resulting in 13–16 amino acid changes as compared to the CAV isolates passaged only 5 times in MSB-1 cells (SMSC-1 and 3-1) (Chowdhury et al., Arch. Virol. 148, 2437–2448, 2003). In this study we found that a low CAV (BL-5) and a high CAV passage (BL-5/P90) differed by only 15 nucleotide substitutions resulting in 11 amino acid changes. Phylogenetic analysis based on VP1 also revealed that both isolates were close to each other but not to other CAV isolates from Malaysia, namely SMSC-1 and 3-1.

Keywords: Chicken anemia virus; virus passaging; nucleotide substitutions; amino acid substitutions; coding regions, nucleotide sequence; phylogenetic analysis
Year: 2004, Volume: 48, Issue:	Page From: 85, Page To: 89


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