Home CONTACT Acta Virologica 2004 Acta Virologica Vol.48, p.167-171, 2004

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Quarterly,
Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

Published in English

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Acta Virologica Vol.48, p.167-171, 2004

Title: TICK-BORNE ENCEPHALITIS VIRUS-SPECIFIC RT-PCR – A RAPID TEST FOR DETECTION OF THE PATHOGEN WITHOUT VIRAL RNA PURIFICATION
Author: N. RUDENKO, M. GOLOVCHENKO, V. CIHLÁŘOVA, L. GRUBHOFFER

Abstract: Among diseases transmitted by ticks in the Czech Republic, tick-borne encephalitis (TBE) caused by Tick-borne encephalitis virus (TBEV) and Lyme disease caused by Borrelia burgdorferi spirochete are most important. We propose an effective and specific test for detection of TBEV in a single tick or a pool of ticks based on the detection of TBEV RNA using an RT-PCR technique without RNA purification. The method is very sensitive with the detection limit of about 14 fg TBEV RNA in total RNA obtained from brain suspension from suckling mice infected with TBEV per reaction. The primers were derived from the 5'-terminal non-coding region, a highly conserved part of the virus. The method was successfully applied to field-collected ticks in detecting TBEV RNA. This method can be used in studies of several aspects of TBEV: epidemiology, screening of natural foci, circulation and detection of virus genome sequences in clinical materials.

Keywords: Ixodes ricinus; Tick-borne encephalitis virus; Chelex® 100 resin; RT-PCR; SYBR Green
Year: 2004, Volume: 48, Issue: Page From: 167, Page To: 171



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