General Physiology and Biophysics Vol.26, p.268-274, 2007

Founded: 1982
ISSN 1338-4325 (online)
ISSN 0231-5882 (print)
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General Physiology and Biophysics Vol.26, p.268-274, 2007
Title: Dehydroepiandrosterone effects on the mRNA levels of peroxisome proliferator-activated receptors and their coactivators in human hepatoma HepG2 cells
Author: H. Poczatkova, K. Bogdanova, L. Uherkova, K. Cervenkova, D. Riegrova, M. Rypka, J. Vesely

Abstract: To investigate the effect of dehydroepiandrosterone (DHEA) on intracellular mRNA levels of peroxisome proliferator-activated receptors (PPARs) and PPAR-γ coactivators (PGCs), we conducted a quantitative real-time RT-PCR study using HepG2 cells. Treatment with 100 μmol/l DHEA for 2–20 h caused a time-dependent elevation of mRNA levels in the cells. Upon 20 h, PPAR-α, -γ1, and -γ2 mRNAs and PGC-1α and -1β mRNAs increased to 157, 161, 155, 656, and 475% of control levels, respectively (p elevated. Cycloheximide also significantly reduced DHEA-induced accumulation of PPAR-α, -γ1, -γ2, and PGC-1α mRNAs, demonstrating the dependence of the DHEA action on de novo protein synthesis. The findings demonstrate that a supraphysiological concentration of DHEA can substantially influence gene expression of the PPAR signalling machinery at both transcriptional and posttranscriptional levels.

Keywords: Dehydroepiadnrosterone — Peroxisome proliferator-activated receptor — PPAR-γ coactivator — mRNA stability — HepG2 cells
Year: 2007, Volume: 26, Issue:	Page From: 268, Page To: 274


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