Home General Physiology and Biophysics 2007 General Physiology and Biophysics Vol.26, p.281-289, 2007

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Founded: 1982
ISSN 1338-4325 (online)
ISSN 0231-5882 (print)
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General Physiology and Biophysics Vol.26, p.281-289, 2007

Title: The activity and isoforms of NADP-malic enzyme in Nicotiana benthamiana plants under biotic stress
Author: V. Doubnerova, A. Jiraskova, M. Janoskova, K. Muller, P. Batkova, H. Synkova, N. Cerovska, H. Ryslava
Abstract: The activity and presence of isoforms of NADP-dependent malic enzyme (NADP-ME, EC 1.1.1.40) were studied in non-transgenic and transgenic Nicotiana benthamiana plants containing potyviral gene for helper component protease (HC-pro) and in plants infected by Potato virus Y strain NTN (PVYNTN). No significant changes in enzyme activities and isoenzyme pattern were observed due to foreign gene introduction and PVYNTN infection. However, the activity and isoenzyme composition of NADP-ME measured in extracts from different parts of the plants showed significant differences. Non-denaturating electrophoresis followed by specific detection of NADP-ME activity in polyacrylamide gel detected the presence of only one isoform in roots and younger leaves. Two isoforms of NADP-ME were detected in older leaves and stem (relative molecular mass ~248,000 and ~280,000) and three isoforms corresponding to tetramer, dimer and monomer were found in flowers. The activity of NADP-ME and the isoenzyme pattern was discussed in relation to its role in plant metabolism within distinct plant parts.
Keywords: NADP-malic enzyme isoforms — Nicotiana benthamiana — HC-pro — Potato virus Y
Year: 2007, Volume: 26, Issue: Page From: 281, Page To: 289


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