Home CONTACT Acta Virologica 2004 Acta Virologica Vol.48, p.173-176, 2004

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Quarterly,
Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

Published in English

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Acta Virologica Vol.48, p.173-176, 2004

Title: A SIMPLIFIED RT-PCR-BASED DETECTION OF RECOMBINANT PLUM POX VIRUS ISOLATES
Author: Z. SUBR, S. PITTNEROVÁ, M. GLASA

Abstract: Closely related natural Plum pox virus (PPV) isolates derived from homologous RNA recombination between PPV-D and PPV-M have been recently identified and shown naturally spread in several European countries. As their serological properties were identical with those of conventional PPV-M isolates, they could be detected only by combined analysis of at least two different genome parts. To simplify the detection of such recombinants primers specific to PPV-M and PPV-D sequences with binding sites located on both sides of the recombination crossover situated in the C-terminal part of NIb were designed. They were used for direct differentiation of PPV-M, PPV-D and their recombinants by reverse transcription–polymerase chain reaction (RT-PCR). This method is convenient for identification of a recombinant PPV in single as well as mixed infection with PPV-M or PPV-D.

Keywords: Sharka; recombination; molecular diagnostic
Year: 2004, Volume: 48, Issue: Page From: 173, Page To: 176



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