Home General Physiology and Biophysics 2008 General Physiology and Biophysics Vol.27, p.59-63, 2008

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Founded: 1982
ISSN  1338-4325 (online)

Published in English

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General Physiology and Biophysics Vol.27, p.59-63, 2008

Title: Angiopoietin-like protein 4: development, analytical characterization, and clinical testing of a new ELISA
Author: D. Stejskal, M. Karpisek, H. Reutova, V. Humenanska, M. Petzel , P. Kusnierova, I. Vareka, R. Varekova, P. Stejskal

Abstract: The aim of our work was to develop an assay for the determination of angiopoietin-like protein 4 (Angplt4) in human blood, and to investigate its levels in healthy volunteers and donors suffer from metabolic syndrome. We developed and evaluated the sandwich ELISA method for the quantitative determination of human Angplt4 in serum samples. We conducted also the pilot study on individuals with metabolic syndrome or familiar hypercholesterolemia and healthy probands and measured blood pressure, waist circumference, Angplt4 serum levels, serum cholesterol, triglycerides, HDL-cholesterol, LDL-cholesterol, insulin, glucose, A-FABP and calculate BMI and QUICKI insulin sensitivity index. In the study on 30 healthy volunteers we demonstrated that sex or age is not the determinant for Angplt4 serum values. Futhermore, we tested 115 individuals with metabolic syndrome and found that probands with metabolic syndrome did not differ in Angplt4 values than healthy individuals from the first study (medians 8.7 vs. 8.1 ng/ml, p = 0.6). Individuals with metabolic syndrome did not differ in sex or age from healthy. Angplt4 values correlated with the HDL-cholesterol (r = –0.25; p

Keywords: Angiopoietin-like protein 4 — ELISA — Metabolic syndrome — Triglycerides
Year: 2008, Volume: 27, Issue: Page From: 59, Page To: 63

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