Neoplasma Vol.52, p.489-493, 2005
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| Title: The effect of doxorubicin on the expression of cyclin A in K-562 leukemia cell line
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| Author: A., GRZANKA
; A., ZURYN
; J., STYCZYNSKI
; A.A., GRZANKA
; H., WISNIEWSKA
; |
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Abstract: Two opposite processes such as apoptosis and cell division cycle
play an important role in cancer biology. During
apoptosis cells die by degrading proteins and genome, whereas in
the cell cycle cells divide and the genome is duplicated.
There are very few studies on the role of cell cycle proteins in
apoptosis although their role is distinctly different from that in
the cell cycle. The significance of expression of cyclin A and
other cyclin cell proteins (eg. Cdk2) in apoptosis remains to be
investigated. The aim of this study was to characterize the
distribution pattern of cyclin A by using the stereptavidin – biotin – peroxidase technique in K-562 cells treated with doxorubicin.
The analysis of cell cycle phases using cytophotometric
methods to estimate the cellular response to doxorubicin was also
used. Studied cells were treated with doxorubicin in the
range 0.5; 5.0 and 10 µM. Expression of cyclin A in K-562 was
32.2; 41.8; 69.9%, respectively, according to doses of
doxorubicin. The number of apoptotic cells was increasing together
with the increase of doxorubicin doses as well as positive
labeling for cyclin A. After doxorubicin treatment decrease of
G1/G0 phase and the growth of cell percentage with dose
dependent manner at G2/M phase, compared to control was observed.
The results allow to suggest that expression of cyclin
A may have pro-apoptotic role however more studies are required to
clarify whether and what role cyclin A plays in
apoptosis.
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| Keywords: cyclin A, K-562 cells, apoptosis, cell cycle, doxorubicin
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| Year: 2005, Volume: 52, Issue: |
Page From: 489, Page To: 493 |
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