Home CUSTOMERS Acta Virologica 2004 Acta Virologica Vol.48, p.193-196, 2004

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Quarterly,
Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

Published in English

Impact Factor = 1.82

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Acta Virologica Vol.48, p.193-196, 2004

Title: EXPRESSION OF CELLULAR PROTEINS BCL-XL , XIAP AND BAX INVOLVED IN APOPTOSIS IN CELLS INFECTED WITH HERPES SIMPLEX VIRUS 1 AND EFFECT OF PAVINE ALKALOID (-)-THALIMONINE ON VIRUS-INDUCED SUPPRESSION OF APOPTOSIS
Author: A. ANGELOVA, T. TENEV, T. VARADINOVA
Abstract: The expression of three cellular proteins involved in the modulation of apoptosis, namely antiapoptotic Bcl-XL and XIAP and proapoptotic Bax, was investigated in cells infected with Herpes simplex virus 1 (HSV-1). To assess whether the regulation of apoptosis in virus-infected cells depends on strain specificity the wild-type (wt) strain Victoria and the mutant R-100 resistant to acyclovir (ACV) were used. In addition, the expression of Bcl-XL, XIAP and Bax was studied in cells infected with HSV-1 and treated with pavine alkaloid (-)-thalimonine. Our previous work has demonstrated that (-)-thalimonine irreversibly inhibits the replication of wt HSV-1 in cultured cells. Our data showed that (-)-thalimonine down-regulates the expression of viral proteins UL17, VP11-12, VP22, VP24 and g134.5, and affects negatively the posttranslational processing of glycoproteins D (gD) and G (gG). As both g134.5 and glycoprotein D possess antiapoptotic activity, we investigated whether the antiviral effect of the alkaloid could also be due to its ability to suppress the antiapoptotic activity of the virus. Our results demonstrated that: (i) the virus induced overexpression of antiapoptotic proteins Bcl-XL and XIAP; (ii) (-)-thalimonine reduced their overexpression, and (iii) this effect was stronger with the acyclovir resistant mutant R-100 than with the wt virus. Taken together, these data suggest that: (i) the virus abolishes apoptosis by means of virus-induced up-regulation of cell-specific prosurvival proteins Bcl-XL and XIAP, and (ii) (-)-thalimonine, apart from affecting essential viral targets, inhibits the infectious progeny production via restoration of apoptosis during viral replication.
Keywords: Herpes simplex virus 1; apoptosis; Bax; Bcl-XL; XIAP; (-)-thalimonine; biological response modifiers
Year: 2004, Volume: 48, Issue: Page From: 193, Page To: 196


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