Neoplasma Vol.50, p.102-109, 2003
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| Title: Lipoxygenase inhibitors suppress proliferation of G5:113 fibrosarcoma cells in vitro but they have no anticancer activity in vivo
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| Author: Z., HOFEROVA
; P., FEDOROCKO
; M., HOFER
; J., HOFMANOVA
; A., KOZUBIK
; V., ELIASOVA
; |
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Abstract: Nordihydroguaiaretic acid (NDGA) and esculetin, both nonspecific inhibitors of
lipoxygenases (LOX), were found to suppress expressively the in vitro
proliferation of fibrosarcoma cells G5:113 in concentrations ranging from 10 to
50 microM. Subsequent flow-cytometric analysis of the cell cycle showed that
both these drugs significantly decreased the percentage proportion of cells in
the G0/G1-phase and simultaneously increased significantly this proportion in
the S-phase. No apoptosis was detected in the whole range of concentrations
studied, from 2.5 to 50 mM. On the contrary, in experiments in vivo, neither
NDGA nor esculetin had any curative effect if they were repeatedly injected
intraperitoneally (i.p.) into mice bearing tumors growing from subcutaneously
(s.c.) transplanted G5:113 cells. Pretreatment of the fibrosarcoma cells with
NDGA or esculetin in vitro preceding their s.c. transplantation into mice did
not result in suppression of the tumor growth, either. Finally, if G5:113 cells
were injected intravenously and the mice were subsequently treated repeatedly
with i.p. injections of NDGA, decreased survival and increased number of surface
lung metastases were observed in the NDGA-treated group. Thus the suppressive
action of inhibitors of LOX on the growth of fibrosarcoma cells in vitro was not
reflected in their anti-tumor effects in vivo.
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| Keywords: fibrosarcoma, lipoxygenase inhibitors.
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| Year: 2003, Volume: 50, Issue: |
Page From: 102, Page To: 109 |
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