Neoplasma Vol.52, p.193-198, 2005
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| Title: Estimation of taxol influence on changes in tubulin and vimentin
systems in K-562 and HL-60 cell lines by immunofluorescence
microscopy
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| Author: A., GRZANKA
; D., GRZANKA
; M., ORLIKOWSKA
; A., ZURYN
; A., GRZANKA
; |
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Abstract: The cytoskeletal system may be considered as an additional pathway
involved in process of apoptosis and can be promising
target for development of new chemotherapies. The study describes
alterations in the distribution of vimentin and
tubulin in taxol treated K-562 and HL-60 cells in relation to
apoptotic changes. K-562 and HL-60 cells were treated with
taxol in a range of concentrations (0.02–10 µM) for 72 hours.
Significant changes in distribution of studied proteins occurred
in the range 2–10 µMof taxol. K-562 cells showed thin network of
vimentin distributed throughout cells or collapsed
on nucleus. There were also cells with bright aggregates
remembering apoptotic bodies. HL-60 cells showed strong labeling
of vimentin in the cytoplasm as well as at the site of apoptotic
bodies. Vimentin collapsed on the nucleus, labeling at poles
and along the major axis of the cell were also seen. K-562 and HL-
60 cells showed radial labeling of tubulin from the centre,
aggregates at the surface and bundled microtubules. These findings
indicate that alterations in expression of studied
cytoskeletal proteins after treatment with taxol were dose-
dependent and related with characteristic features of apoptosis.
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| Keywords: taxol, K-562 cells, HL-60 cells, immunofluorescence microscopy,
vimentin, tubulin, apoptosis
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| Year: 2005, Volume: 52, Issue: |
Page From: 193, Page To: 198 |
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