Home CONTACT Acta Virologica 2004 Acta Virologica Vol.48, p.223-227, 2004

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Quarterly,
Founded: 1957
ISSN 0001-723X
E-ISSN 1336-2305

Published in English

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Acta Virologica Vol.48, p.223-227, 2004

Title: ISOLATION OF BOVINE VIRAL DIARRHEA VIRUS 1, A PESTIVIRUS FROM AUTOPSIED LAMB SPECIMEN FROM TAMIL NADU, INDIA
Author: P. YADAV, P.V. BARDE, R. JADI, M.D. GOKHALE, A. BASU, M.V. JOSHI, R. MEHLA, S.R.P. KUMAR, S.S. ATHAVALE, D.T. MOURYA

Abstract: An epizootic of febrile illness among the Madras red breed of sheep had occurred in 1994 in Verrapuram, Chennai, India. The epizootic was suspected as Rift Valley fever (RVF)-like sickness based on clinical features. However, its etiological agent could neither be isolated nor implicated conclusively. During the post-epizootic period a male lamb died of similar clinical features and the spleen was immediately collected. Inoculation of spleen suspension in infant mouse brain yielded a virus that was serially passaged in infant mice and rhabdomyosarcoma (RD) cells. Electron microscopic observations revealed virus particles resembling flaviviruses. RT-PCR performed on extracted total RNA from infected cells and mouse brains with flavivirus-specific or RVF-specific primers gave negative results. However, an amplicon of 280 bp was obtained with pestivirus-specific primers from the 5'-UTR. Further, a nested PCR yielded a product of 157 bp. Nucleotide sequencing of the 157 bp product showed 100% homology to BVDV-1. Western blot analysis with a flavivirus envelope protein-specific MAb revealed three proteins of 33 K, 45 K and 55 K. Further studies suggested that the 33 K and 55 K proteins were glycosylated. This is the first report of isolation of BVDV-1 from a lamb in India.

Keywords: Bovine viral diarrhea virus 1; flavivirus; nested PCR; nucleotide sequencing; pestivirus; RT-PCR; Rift Valley fever; virion morphology; Western blot analysis
Year: 2004, Volume: 48, Issue: Page From: 223, Page To: 227



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