Neoplasma Vol.52, p.137-142, 2005
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| Title: Significance of qualitative PCR detection method for preemptive
therapy of cytomegalovirus infection in patiens after allogeneic
hematopoietic stem cell transplantation – single-centre experience
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| Author: D., SKAPOVA
; Z., RACIL
; D., DVORAKOVA
; D., MINARIKOVA
; J., MAYER
; |
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Abstract: Both early cytomegalovirus (CMV) monitoring and prophylactic
antiviral therapy can decrease clinical complications or can
prevent them in patients after allogeneic hematopoietic stem cell
transplantation (HSCT). Presented paper summarizes experiences
with using regular monitoring of reactivation of CMV after
allogeneic HSCT by qualitative polymerase chain reaction (PCR)
method to prevent the development of symptomatic CMV disease.
Samples of peripheral blood leukocytes (PBL) in 71 patients were
monitored. Because of retransplantations in two patients, 73
transplantations, each followed by the monitoring, were performed.
Patients were monitored weekly after the transplantation for CMV
DNAemia in PBL. An episode of CMV infection representing an
indication for pre-emptive ganciclovir (GCV) or foscarnet (FOS)
therapy was defined as two consecutive positive PCR results in 4–7
days. Median time of monitoring was 313 days. The CMV infection
was found in 28/73 monitorings (38.4 %) and always was followed by
pre-emptive therapy. One recurrence of CMV infection was observed
in 4/28 (14.3 %) monitorings and two recurrences in 1/28 (3.6 %)
monitorings. Presented approach resulted in complete prevention of
overt CMV disease and this study enable to show that qualitative
PCR method for determination of incipient CMV infection followed
by pre-emptive therapy is suitable for preventing patients after
allogeneic transplantation from CMV disease.
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| Keywords: cytomegalovirus, polymerase chain reaction, allogeneic
hematopoietic stem cell transplantation
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| Year: 2005, Volume: 52, Issue: |
Page From: 137, Page To: 142 |
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