Journal info
|
||
Select Journal
Journals
Bratislava Medical Journal Endocrine Regulations General Physiology and Biophysics Neoplasma 2024 Ahead of print 2023 2022 2021 2020 2019 2018 2017 2016 2015 2014 2013 2012 2011 2010 2009 2008 2007 2006 2005 2004 2003 Acta Virologica Studia Psychologica Cardiology Letters Psychológia a patopsych. dieťaťa Kovove Materialy-Metallic Materials Slovenská hudbaWebshop Cart
Your Cart is currently empty.
Info: Your browser does not accept cookies. To put products into your cart and purchase them you need to enable cookies.
Neoplasma Vol.52, p.211-218, 2005 |
||
Title: Myeloid enzymes profile related to the immunophenotypic characteristics of blast cells from patients with acute myeloid leukemia (AML) at diagnosis | ||
Author: M., KLOBUSICKA ; J., KUSENDA ; O., BABUSIKOVA ; | ||
Abstract: The purpose of this study was to assess the possible relationship
between the cytochemical enzyme profile and
immunophenotypic characteristics of distinct acute myeloid
leukemia (AML) subtypes in discrete stages of leukemic cells
maturation. As the proportion of leukemic blast cells is critical
for exact cytochemical analysis, study was restricted to the
evaluation of 48 adult and pediatric patients with newly diagnosed
AMLs with 80% or more blasts in analyzed samples. The
cytochemical investigation of myeloperoxidase (MPO), Sudan black B
(SBB), chloroacetate esterase (CAE), alpha-naphthyl
butyrate esterase (ANBE), alpha-naphthyl acetate esterase (ANAE) and
acid phosphatase (AP) in peripheral blood and/or bone
marrow was performed. The immunophenotype was examined for the
maturation dependent myeloid antigens CD13,
CD33, CD11b, CD14, CD15, CD65, CD36, cytoplasmic MPO, non-lineage
associated CD34 and HLA-DR antigens, lymphoid-
associated antigens CD7, CD4, CD38 as well as natural killer cell
associated marker CD56. Flow cytometry by double
marker staining and visualization of pathologic cells in dot plots
reflected immunophenotypic aberrancy and degree of
cell maturation. The patients were classified into AML subtypes M0-
M2, M3, M4 and M5 according to the main morphological,
cytochemical and immunophenotypical features. The variable
combinations of MPO, SBB, CAE and ANBE were
identified in relation to immunophenotype. The cytochemical
profile of blasts was in concordance with immunophenotype,
particularly in more differentiated AML subtypes, M3, M4 and M5.
The findings of myeloid antigens expression and
cytochemical features in poorly differentiated AML subtypes showed
no practical relevance of cytochemical analysis. Notwithstanding
that the cytochemical analysis of AML subtypes not sufficiently
identifies the distinct aberrancies in heterogeneous
leukemic blast cell populations, evaluation of the cytochemical
profile in connection with immunophenotyping may
help to classify the AML patients to relevant subtypes with more
accuracy.
|
||
Keywords: acute myeloid leukemia, immunophenotype, cytochemistry, myeloid enzymes | ||
Year: 2005, Volume: 52, Issue: | Page From: 211, Page To: 218 | |
|
download file |
|