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Neoplasma Vol.53, p.402-409, 2006 |
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Title: Mung bean sprout (Phaseolus aureus) nuclease and its biological and antitumor effects | ||
Author: J., SOUCEK ; J., SKVOR ; P., POUCKOVA ; J., MATOUSEK ; T., SLAVIK ; J., MATOUSEK ; | ||
Abstract: Bovine seminal ribonuclease (BS RNase), a dimeric homolog of
bovine pancreatic ribonuclease (RNase A), is known to
display special biological activities namely cytotoxicity for
human tumor cells. Because some plant ribonucleases have a
similar mass weight and structure as the animal ribonuclease,
effects of a commercial product of Mung bean (Phaseolus
aureus) nuclease (PhA) were studied on proliferation of ML-2 human
tumor cells, as well as it’s aspermatogenic,
embryotoxic, immunogenic, and immunosuppressive activity, and
therapeutic efficiency in athymic mice bearing human
melanoma tumor. Concerning the antiproliferative activity, PhA
nuclease was almost non-effective in vitro on ML-2 cells
and also immunosuppressive activity on human lymphocyte in mixed
culture was very low compared to that of BS RNase.
However, significant antitumor activity was detected on human
melanoma tumor after intratumoral or intraperitoneal administration
into the mice. Furthermore conjugate of PhA nuclease with
polyethylen glycol (PEG) injected seven times at
the dose of 10 µg intraperitonealy showed identical antitumor
activity as that of bovine seminal ribonuclease (BS RNase) injected
by the same way at ten times higher dose.
Both PhA and BS RNases exerted strong aspermatogenic effect on the
width of spermatogenic layers while RNase A administration
at ten times higher concentration was ineffective. PhA nuclease
when compared by means of antibody cross reaction
with RNase A, BS RNase and wheat leaf neutral RNase (WLN-RNase)
was found to be immunologically similar to
RNase A and WLN-RNase, meanwhile BS RNase showed much higher
antigenicity in comparison with them.
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Keywords: plant ribonuclease, aspermatogenesis, embryotoxicity, antitumor effect, melanoma, mice | ||
Year: 2006, Volume: 53, Issue: | Page From: 402, Page To: 409 | |
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