Neoplasma Vol.52, p.208-210, 2005
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| Title: Comparative analysis of thymidylate synthase, thymidine
phosphorylase and dihydropyrimidine dehydrogenase expression in
colorectal cancer and surrounding normal tissue
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| Author: M., ZIMOVJANOVA
; V., SYKORA
; J., NOVOTNY
; J., GATEK
; L., PETRUZELKA
; L., HOLUBEC
; L., PECEN
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Abstract: Thymidylate synthase [TS], thymidine phosphorylase [TP] and
dihydropyrimidine dehydrogenase [DPD] play the essential
role in the activation and catabolism of the fluoropyrimidines
used in cancer therapy. It’s expression may influence the
antitumor activity or toxicity of these drugs. We studied the
expression levels of selected enzymes in colorectal tumors and
adjacent normal mucosa.
The analysis of TS, TP and DPD gene expression was performed using
quantitative Real time PCR technique (Roche) in
15 (TS), 64 (TP) and 12 (DPD) of 64 colorectal cancer patients.
The mean gene expression of TS, TP and DPD was found to be 3.29;
3.79 and 8.24 in tumors and 1.88; 3.80 and 19.69 in
normal mucosa. The corresponding median gene expression was 1.87;
2.32 and 4.50 for tumors and 2.14; 2.63 and 11.64 for
normal tissue.
We did not find any significant differences in TS, TP and DPD gene
expression between colorectal tumor and surrounding
mucosa.
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| Keywords: thymidylate synthase, thymidine phosphorylase, dihydropyrimidine
dehydrogenase, colon cancer
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| Year: 2005, Volume: 52, Issue: |
Page From: 208, Page To: 210 |
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