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Neoplasma Vol.51, p.345-351, 2004 |
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Title: The value of multiparameter flow cytometry of cerebrospinal fluid involved by leukemia/lymphoma cells | ||
Author: O., BABUSIKOVA ; T., ZELEZNIKOVA ; | ||
Abstract: The usefulness of multiparameter flow cytometric (FC) analysis of
cerebrospinal fluid (CSF) was evaluated in leukemia/lymphoma
patients having central nervous system (CNS) involvement of the
disease. In 12 specimens of 8 patients with different types of
leukemia/lymphoma (one case of T-ALL, 3 cases of early B-cell ALL,
one case of AML, and 3 proven or suspicious NHL cases) the
presence of pathological clone in CSF has been confirmed or
excluded. The phenotypic patterns of CSF cells were defined
according to those of bone marrow (BM)/peripheral blood (PB) at
diagnosis or during follow-up of the same patients. Furthermore,
in one case of suspicious CNS infiltration of NHL, the
pathological clone was characterized as a highly suspicious of
solid tumor and was proved to be a lung cancer metastasis. The
definition was made on the basis of CD45 (common leukocyte
antigen) and other studied CD markers negativity. The exact
comparison of immunophenotypic profiles of specimens from
different sites (CSF, BM, PB) of the same patient has been
performed and no phenotypic changes were found. In some CSF
specimens, where no cells of suspicious pathological clone were
detected, in 4-color analysis only normal lymphocyte population
was found even in small cell samples (even if the cellularity was
< than 0.3x10-6). In these populations the high values of T-cells
(CD3+) predominated and the high prevalence of CD4+ over CD8+
cells, and an almost total lack of B-lymphocytes was found.
Our results suggest that positive CSF immunology is a useful
indicator of malignancy and reflects leptomeningeal involvement.
Simultaneously we demonstrated that FC analysis of CSF in the aim
to detect possible CSF seeding of leukemia/lymphoma is a reliable
and quick technique.
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Keywords: flow cytometry, multiparameter analysis, cerebrospinal fluid, lymphocyte subpopulations, CNS tumors | ||
Year: 2004, Volume: 51, Issue: | Page From: 345, Page To: 351 | |
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